RESUMO
BACKGROUND: The prevalence of hearing impairment in patients with diabetes was significantly higher, and the development of preventive methods is desirable. AIMS/OBJECTIVES: This study examined the effects of eicosapentaenoic acid (EPA) administration on the prevention of early hearing loss in diabetic mice. MATERIALS AND METHODS: Tsumura, Suzuki, Obese Diabetes (TSOD) mice were used as a model of diabetes and Tsumura, Suzuki, Non Obesity (TSNO) mice were used as controls. The animals were divided into three groups: the TSNO group and the TSOD (EPA-) group (provided sunflower oil), and the TSOD (EPA+) group (provided EPA). Auditory brainstem responses (ABRs) were measured and the cochlea was evaluated histologically. RESULTS: The TSOD (EPA+) group showed a lower tendency to increase thresholds than the TSOD (EPA-) group. The TSOD (EPA+) group had a significantly lower ABR threshold than the TSOD (EPA-) group from 11 to 14 months of age at 4 kHz. Narrowing of the capillary lumens in the stria vascularis and thickening of the vessel wall in the modiolus were observed in the TSOD (EPA-) group. CONCLUSIONS AND SIGNIFICANCE: It was suggested that the suppression of cochlear vascular atherosclerosis by EPA administration in TSOD mice suppressed early age-related hearing loss.
Assuntos
Surdez , Diabetes Mellitus Experimental , Diabetes Mellitus Tipo 2 , Perda Auditiva , Camundongos , Animais , Ácido Eicosapentaenoico/farmacologia , Ácido Eicosapentaenoico/uso terapêutico , Diabetes Mellitus Experimental/complicações , Diabetes Mellitus Experimental/patologia , Perda Auditiva/etiologia , Perda Auditiva/prevenção & controle , Modelos Animais de Doenças , Obesidade , Cóclea/patologia , Camundongos ObesosAssuntos
Amiloidose/tratamento farmacológico , Amiloidose/etiologia , Artrite Reumatoide/complicações , Artrite Reumatoide/tratamento farmacológico , Úlceras Orais/tratamento farmacológico , Úlceras Orais/etiologia , Idoso , Anti-Inflamatórios/uso terapêutico , Antirreumáticos , Feminino , Humanos , Metotrexato/uso terapêutico , Prednisolona/uso terapêuticoRESUMO
OBJECTIVE: The temporal fascia has been widely used in tympanoplasty. In addition, the preserved fascia has been also used in the ear surgery. In this study, we planned the experiments to determine whether physical and chemical properties of the fascia preserved at a low temperature. METHODS: Preserved temporal fasciae from 21 patients were used in this study. The thickness of the temporal fascia was measured under a 3D laser microscope. The tensile strength was evaluated using a tensile tester. In addition, the chemical property evaluated was the biologic antioxidative potential of samples. RESULTS: The results showed that the strength of the fascia was not affected by the retention period. The thick fascia tended to show the less tensile strength. The intensity was highest in middle-aged donors when compared to young and older donor. The antioxidative potentials did not affect the preservation. CONCLUSION: The results suggested that the preserved temporal fascia could be safely used for tympanoplasty.
